Studies on Histidyl Peptides. I. Imidazole Protected Histidine in the Synthesis of C-Terminal and N-Terminal Histidyl Peptides.

The influence of histidine on cleavage C-terminal to acidic residues in doubly protonated tryptic peptides

An ion-trap CID MS/MS spectral database of 505 doubly protonated tryptic peptides was used to investigate the influence of an internal basic residue on preferential fragmentation C-terminal to the acidic amino acid residues, aspartic acid (Asp) and glutamic acid (Glu). Because tryptic peptides, which contain C-terminal Lys or Arg, were selected for analysis, the majority of the peptides contain...

N-terminal POMC peptides and adrenal growth

The peptide hormones contained within the sequence of proopiomelanocortin (POMC) have diverse roles ranging from pigmentation to regulation of adrenal function to control of our appetite. It is generally acknowledged to be the archetypal hormone precursor, and as its biology has been unravelled, so too have many of the basic principles of hormone biosynthesis and processing. This short review f...

The terminal peptides of insulin.

cystatin c, n-terminal probrain natriuretic peptides t-terminal probrain natriuretic peptides and outcomes in acute heart failure with acute kidney injury in a 12-month follow-up: insights into the cardiorenal syndrome

background: cystatin c (cys c) has been implicated as a prognostic marker in cardiovascular disease. the aim of this study was to evaluate the value of cys c as a marker of acute kidney injury (aki) in acute heart failure (ahf), the impact of cys c and n-terminal probrain natriuretic peptides (nt-probnp) on in-hospital and 12 months mortality were also investigated. materials and methods: a tot...

Selective N-terminal functionalization of native peptides and proteins.

We report an efficient, highly selective modification on the N-terminal amines of peptides and proteins using aldehyde derivatives via reductive alkylation. After modification of a library of unprotected peptides XYSKEASAL (X varies over 20 natural amino acids) by benzaldehyde at room temperature, pH 6.1 resulted in excellent N-terminal selectivity (α-amino/ε-amino: >99 : 1) and high reaction c...